He wants to avoid a repeat of 2002 when Rita Montella, wife of Vincenzo, smuggled a video camera into the hotel to record a diary for a TV football show.
The Gospel according to Trapattoni, who has not had his contract renewed and is "enthusiastic" about managing Tottenham, was handed to the 23-man Italy squad at their training camp at Coverciano near Florence.
An Italian FA source said: "Signor Trapattoni is determined to win the tournament and as such has demanded the utmost commitment from his squad.
"He strongly believes in (Luis Felipe) Scolari's saying that whoever cannot go without sex for 40 days is an animal, and Brazil went onto win the World Cup."
It is a complete reversal of England's set-up. The squad have travelled to their training camp in Sardinia with wives, girlfriends and children in tow.