Lee has used the skills and knowledge of two Wimbledon champions to prepare him for today's clash, working out with Agassi and then practising with 1996 winner Richard Krajicek.
"Agassi was complimentary,"-said Lee, "he was pleased with the way I moved. I am fast about the court, and movement against Sampras will be very important."
Lee, who as a wild-card entry last year went out to Tim Henman in the second round on the Centre Court, is now glad of that experience.
"The first time out there was daunting. I will speak to Tim about Sampras because he has played him so many times.
" Everyone knows what Sampras's strengths are, and I know he will be looking for a high standard from the start. But I am looking forward to the challenge."
Sampras has won titles under the threat of injury, including his last Wimbledon crown two years ago.
Meanwhile Greg Rusedski, the British No 2 and a quarter-finalist in 1997, begins on Court No 1 against Jurgen Melzer of Austria.