Jude Bellingham, who is set to be the last player to join up with the squad after helping Real Madrid to win another European Cup at the weekend, is a shoo-in, while Phil Foden is likely to start from the left but can also play centrally.
With Palmer expected to make the cut after his outstanding season for Chelsea, there will likely only be space for two of Eze, Grealish or Maddison, and the Palace forward, though the least experienced, is plainly the form player.
After being ignored when Manchester City needed a goal in their FA Cup Final defeat, Grealish offered a timely reminder of his own quality, replacing Eze for the final half-hour and creating Alexander-Arnold's superb finish with a lovely switch of play.
Grealish and Maddison were both involved in the third goal, scored by Kane, who bundled home from close-range, having earlier asked Southgate if he could come on to take the penalty, won by Konsa, following a VAR review.
"It's a 33 who are desperate to be involved," Southgate said, when asked about Grealish. "We know the qualities he has and what he's capable of doing. That's not in any question. He's a player we love having with the group and a great character to work with."
Southgate could yet take all three of Eze, Grealish and Maddison, particularly if Anthony Gordon continues to struggle for fitness, but the Newcastle winger and Jarrod Bowen, who completed 90 minutes on the right wing, offer more direct options.
If there was a concern for Southgate, it was in a familiar area of the pitch, as Eze's Palace team-mate Marc Guehi looked jittery at centre-half.
In the past 18 months, Guehi has established himself as first change when either Harry Maguire or John Stones is sidelined, but he was loose in possession and booked for a clumsy challenge. Konsa, who played in every position across the back four last night, may have jumped ahead of him in the pecking order.
Next up for England is Iceland on Friday and, having run the rule over his squad last night, Southgate is likely to prioritise to fine-tuning his preferred starting XI at Wembley.
