The Duchess of Cornwall has been the trust's patron since 2010 and opened the base in Newquay two years later.
The trust is the oldest air ambulance in the UK after launching 33 years ago.
Its team is called to more than 1,000 seriously sick or injured people every year in Cornwall and the Isles of Scilly.
During Covid-19, the Air Ambulance and Critical Care Cars have continued to fly. But the closure of their charity shops, local schools, businesses and tourism, has hit the trust's fundraising and finances.
The Duchess’s helicopter went into service on April 1.
New Heli Appeal, the public fundraising campaign raised over £2.7 million to help bring the new aircraft to Cornwall, and the remainder came from the Trust's reserves.
The new helicopter was designed by the charity and locals and boasts red with yellow chevrons.
It has St Piran’s flag and Cornish language prominent features on the livery. It is the first time the charity has owned and not leased its own helicopter.