Harriet Williams, from PSBA, said: "The only true trial involved here will be that endured by local residents, as they face a year of weeds growing upon streets and pavements, and the smell of vinegar in unexpected places."
Gillian Douglas, from Bristol City Council, said: "This trial of Glyphosate-free weed treatments is still in its early stages, having been implemented in response to widespread public concern about the use of Glyphosate as a weed killer.
"We are sorry to hear that some residents are concerned about the use of vinegar and we will monitor feedback throughout the trial."
Earlier this month MEPs voted for a ban on glyphosate being used by the public.