Negotiations will start between LU management and train drivers' unions on Thursday on Night Tube terms. An extra 85 drivers are required and 180 station staff, many part-time.
Finn Brennan of Aslef said: "LU are reducing the number of part time staff who work during peak hours Monday to Friday. Those positions are being got rid of and instead they will recruit station staff who will work Saturdays and Sunday nights which will have a major impact on work-life balance."
The Night Tube announcement comes as the RMT starts an overtime ban in protest at Tube ticket office closures, resulting in almost 1000 job losses.
Mick Cash, RMT General Secretary, said: "the night tube plans are not properly thought through, are being used to divert attention from the massive cuts programme and are a disaster waiting to happen."
The Metropolitan, District, Hammersmith & City and Circle lines are due next to go 24-hour although this will not until 2019 as LU sorts out a bungled signalling upgrade.
Mr Brown, who shortly takes control of Crossrail, would also operate 24-hours eventually.
London mayor Boris Johnson said: “London is a bustling, 24-hour global city and by this time next year we’ll have a 24-hour Tube service to match. Running trains all through the night was once thought impossible, but with the huge investment we’ve put in and upgrades that have been delivered we stand ready to take the Tube to the next level."