Mayor of London Boris Johnson said the lives of cyclists and pedestrians would be "much safer" under the new rules.
He said: "We know that a large number of cyclist deaths and serious injuries involve a relatively small number of trucks and lorries that are not fitted with basic safety equipment.
"Such vehicles are not welcome in the capital and the Safer Lorry Scheme will see them effectively banned from our streets.
"The lives of thousands of cyclists and pedestrians will be much safer as a result and I urge all operators of HGVs to get on board and make it a success."